Conclusion: The presented method proved to be a simpler and cheaper way of processing whole-blood samples for PCR and RFLP analyses. In molecular biology, restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms, populations, or species or to pinpoint the locations of genes within a sequence. Molecular marker Method of RFLP Various applications of RFLP Advantages and Disadvantages of RFLP. Results: PCR/RFLP results using the presented method were qualitatively similar to those obtained by DNA extracted using the other three methods. For comparison, PCR and RFLP were performed on DNA purified from the same samples using the phenol-chloroform method and two commercial DNA extraction kits. Restriction fragment length polymorphism (RFLP) analysis was used to characterize variability in the Illinois Long-Term Selection Experiment oil strains. The supernatant was taken directly for PCR and RFLP. Each sample was incubated with distilled water, then boiled in a microwave and centrifuged. The samples were frozen at –80☌, and then rapidly thawed at 37☌. of DNA fingerprinting by restriction fragment length polymorphism (RFLP) for. These can be separated by electrophoresis, with the smaller fragments migrating farther than the larger fragments. Results from this study suggest that RFLP analysis may be of value in allocating maize inbreds to heterotic groups, but no rela- tionship between RFLP-based. Materials and Methods: Venous whole-blood samples were collected from 40 individuals. Introduction of pharmaceutical chemistry, General and safety rules for. Restriction enzymes cut DNA at precise points producing a collection of DNA fragments of precisely defined length. Objectives: The aim of the present study was to develop a simple, quick and cheap method to process whole-blood samples for the molecular techniques polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) without the use of expensive reagents or sophisticated machines.
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